Issue 14, 2018, Issue in Progress

Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes

Abstract

We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.

Graphical abstract: Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes

Supplementary files

Article information

Article type
Paper
Submitted
15 Dec 2017
Accepted
10 Feb 2018
First published
16 Feb 2018
This article is Open Access
Creative Commons BY license

RSC Adv., 2018,8, 7514-7517

Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes

H. J. Lee, G. H. Go, J. J. Ro and B. H. Kim, RSC Adv., 2018, 8, 7514 DOI: 10.1039/C7RA13349A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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