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AIE-based super-resolution imaging probes for β-amyloid plaques in mouse brain

Abstract

Fluorescence microscopy is an intuitive detection method of β-amyloid (Aβ) fibrillation which usually occur in early-stage Alzheimer's disease. By the aid of single-molecular localization of reversibly-activated fluorogens, the limit of optical diffraction in fluorescent microscopy can be broken through and the imaging resolution can be promoted to sub-100 nm boundary. Aggregation-induced emission (AIE) is the fluorogenic emission behavior in which the fluorescence is significantly enhanced due to the restriction of intramolecular motion when the fluorogens aggregate together or bind with specific targets. Reversible binding events of AIE-active fluorogens cause fluorescence switching enabling single-molecular localization and super-resolution imaging. Here, we report a series of super-resolution fluorescent probes with AIE activity, which are used for detection and super-resolution imaging of fibrillar amyloid. The AIE-active fluorogens show superior in vitro sensitivity to fibrillar amyloid of hen egg white lysozyme (HEWL), which is usually used as a model protein for amyloid studies, with the limit of detection down to 63.71 nM. The fluorescence colocalization imaging indicates the excellent ex vivo targeting capability of Aβ plaques in mouse brain slices, with the colocalization degrees more than 90%. Based on reversible binding between AIE-fluorogens and Aβ fibrils, AIE-based super-resolution imaging of in vitro Aβ fibrillation in tubes and ex vivo Aβ plaques in mouse brain slices is accomplished. The detailed structure information is unveiled that Aβ plaques in mouse brain are composed of numerous radiant nanofibrils with optical imaging resolution of about 30 nm.

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Publication details

The article was received on 04 May 2018, accepted on 13 Jun 2018 and first published on 14 Jun 2018


Article type: Research Article
DOI: 10.1039/C8QM00209F
Citation: Mater. Chem. Front., 2018, Accepted Manuscript
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    AIE-based super-resolution imaging probes for β-amyloid plaques in mouse brain

    Y. Wang, C. Fan, B. Xin, J. Zhang, T. Luo, Z. Chen, Q. Zhou, Q. Yu, X. Li, Z. Huang, C. Li, M. Zhu and B. Z. Tang, Mater. Chem. Front., 2018, Accepted Manuscript , DOI: 10.1039/C8QM00209F

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