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Issue 14, 2018
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RAFT polymerization of a RGD peptide-based methacrylamide monomer for cell adhesion

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Abstract

Synthetic peptides containing the arginine–glycine–aspartate (RGD) motif have been used extensively as inhibitors of integrin–ligand interactions in studies of cell adhesion and cell target moiety. MARGD (sequences: Phe-Gly-Arg-Gly-Asp-Ser), a methacrylamide monomer containing a pending RGD peptide, was synthesized and obtained in high purity (≥90%) via an automated peptide synthesizer followed by a simple precipitation in diethyl ether. MARGD can be polymerised by reversible addition–fragmentation chain transfer (RAFT) polymerization to afford well-defined polymers containing a RGD peptide group with controlled molecular weight, low dispersity (Đ < 1.25), and a precise chain end structure. In this study, linear pseudo-first-order kinetics and number average molecular weight dependence on conversion were observed during the RAFT polymerization. Diblock peptide-polymer conjugates were prepared using PMARGD as a macro-chain transfer agent or using MARGD as a monomer, and the resulting diblock conjugates all showed low dispersities. The cytotoxicity study using mouse fibroblast cells (L929) revealed that the bioconjugates are non-toxic up to high concentration. Furthermore, enhanced cell adhesion was observed when the bioconjugates immobilized on the glass slide surfaces. This study provides a novel and efficient strategy to access well-defined peptide-polymer conjugates with diversity for both peptides and polymers.

Graphical abstract: RAFT polymerization of a RGD peptide-based methacrylamide monomer for cell adhesion

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Publication details

The article was received on 10 Nov 2017, accepted on 16 Feb 2018 and first published on 20 Feb 2018


Article type: Paper
DOI: 10.1039/C7PY01887H
Citation: Polym. Chem., 2018,9, 1780-1786
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    RAFT polymerization of a RGD peptide-based methacrylamide monomer for cell adhesion

    C. Chen and S. H. Thang, Polym. Chem., 2018, 9, 1780
    DOI: 10.1039/C7PY01887H

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