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Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of Caspase 3 and Bcl-2 genes in acute lung injury

Abstract

Acute respiratory distress syndrome (ARDS) and acute lung injury (ALI) are defined as a pulmonary inflammation, could occur from sepsis and leads to pulmonary permeability and alveolar edema making it a life-threatening disease. Photobiomodulation (PBM) properties have been widely described in the literature in several inflammatory diseases; although the mechanisms of action are not always clear, this could be a possible treatment for ARDS/ALI. Thus, the aim of this study was to evaluate the mRNA levels from Caspase-3 and BCL-2 genes and DNA fragmentation in lung tissue from Wistar rats affected by ALI and submitted to photobiomodulation by exposure to low power infrared laser (808nm; 100mW; 3.571W/cm²; four points per lung). Adult male Wistar rats were randomized into 6 groups (n=5, for each group): control, PBM10 (10J/cm², 2J and 2 seconds), PBM20 (20J/cm², 5J and 5 seconds), ALI, ALI+PBM10 and ALI+PBM20. ALI was induced by intraperitoneal Escherichia coli lipopolysaccharide injection. Lung samples were collected and divided for mRNA expression of Caspase-3 and Bcl-2 and DNA fragmentation quantifications. Data show that Caspase-3 mRNA levels are reduced and Bcl-2 mRNA levels increased in ALI after low power infrared laser exposure when compared to non-exposed ALI group. DNA fragmentation was increased in inflammatory infiltrate cells and reduced in alveolar cell. Our research shows that photobiomodulation can alter mRNA relative levels from genes involved in apoptotic process and DNA fragmentation in inflammatory and alveolar cells after lipopolysaccharide-induced acute lung injury. Also, inflammatory cell apoptosis is part of the photobiomodulation effects induced by exposure to low power infrared laser.

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Publication details

The article was received on 14 Mar 2018, accepted on 04 Jun 2018 and first published on 07 Jun 2018


Article type: Paper
DOI: 10.1039/C8PP00109J
Citation: Photochem. Photobiol. Sci., 2018, Accepted Manuscript
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    Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of Caspase 3 and Bcl-2 genes in acute lung injury

    L. P. S. Sergio, A. M. C. Thomé, L. A. D. S. N. Trajano, A. L. Mencalha, A. S. Fonseca and F. Paoli, Photochem. Photobiol. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8PP00109J

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