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Issue 4, 2018
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Photodynamic effect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis

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Abstract

Leishmaniasis is a neglected disease present in more than 88 countries. The currently adopted chemotherapy faces challenges related to side effects and the development of resistance. Photodynamic therapy (PDT) is emerging as a therapeutic modality for cutaneous leishmaniasis. Zn(II) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (ZnTE-2-PyP4+, ZnP) is a cationic, water-soluble, zinc porphyrin-based photosensitizer whose photodynamic effect on Leishmania braziliensis was analyzed by evaluating the number of visibly undamaged and motile cells, cell membrane integrity, mitochondrial membrane potential, and ultrastructural damage. Treatment of parasites with ZnP and light induced damage in up to 90% of L. braziliensis promastigote cells. Propidium iodide labeling suggested the loss of plasma membrane integrity. In samples treated with ZnP and light, a hyperpolarization of the mitochondrial membrane potential was also observed. Ultrastructural evaluation of promastigotes after photodynamic treatment indicated a loss of cytoplasmic material and the presence of vacuoles. Scanning electron microscopy showed wrinkling of the plasma membrane and a reduced cell volume. Additionally, the number of amastigotes per macrophage was reduced by about 40% after photodynamic application. The treatment showed no considerable toxicity against mammalian cells. Therefore, the results indicated that PDT associated with ZnTE-2-PyP4+ represents a promising alternative to cutaneous leishmaniasis treatment.

Graphical abstract: Photodynamic effect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis

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Publication details

The article was received on 20 Dec 2017, accepted on 27 Feb 2018 and first published on 27 Mar 2018


Article type: Paper
DOI: 10.1039/C7PP00458C
Citation: Photochem. Photobiol. Sci., 2018,17, 482-490
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    Photodynamic effect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis

    C. G. Andrade, R. C. B. Q. Figueiredo, K. R. C. Ribeiro, L. I. O. Souza, J. F. Sarmento-Neto, J. S. Rebouças, B. S. Santos, M. S. Ribeiro, L. B. Carvalho and A. Fontes, Photochem. Photobiol. Sci., 2018, 17, 482
    DOI: 10.1039/C7PP00458C

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