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Energy Transfer Chemiluminescence for Ratiometric pH Imaging

Abstract

Chemiluminescence imaging offers a low background and high sensitivty approach to imaging analytes in living cells and animals. Intensity-based measurements have been developed, but require careful consideration of kinetics, probe localization, and fluctuations in quantum yield, all of which complicate quantification. Here, we communicate a ratiometric strategy for quantitative chemiluminescence imaging of pH. The strategy relies on an energy transfer cascade of chemiluminescence emission from a spiroadamantane 1,2-dioxetane to a ratiometric pH indicator via fluorescent dyes in Enhancer solutions. Monitoring the pH-dependent changes in chemiluminescence emission at multiple wavelengths enables ratiometric imaging and quantification of pH independent from variations due to kinetics and probe concentration.

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Publication details

The article was received on 25 Apr 2018, accepted on 17 May 2018 and first published on 17 May 2018


Article type: Paper
DOI: 10.1039/C8OB00972D
Citation: Org. Biomol. Chem., 2018, Accepted Manuscript
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    Energy Transfer Chemiluminescence for Ratiometric pH Imaging

    W. An, R. Mason and A. Lippert, Org. Biomol. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8OB00972D

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