Issue 37, 2018

Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

Abstract

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.

Graphical abstract: Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

Supplementary files

Article information

Article type
Communication
Submitted
08 Feb 2018
Accepted
07 Mar 2018
First published
07 Mar 2018

Org. Biomol. Chem., 2018,16, 8245-8248

Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

S. E. Hagen, K. Liu, Y. Jin, L. Piersimoni, P. C. Andrews and H. D. Showalter, Org. Biomol. Chem., 2018, 16, 8245 DOI: 10.1039/C8OB00329G

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