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Targeted imaging of lysosome and endoplasmic reticulum and their pH monitoring with surface regulated carbon dots

Abstract

Organelles play crucial roles in cellular activities and the functions of organelles are related greatly to the pH values, therefore, the bio-imaging of targeted organelles and related pH sensing is of great importance in biological assays. Herein we report the fluorescent imaging of specific organelles, i.e., lysosome and endoplasmic reticulum, and their pH sensing with surface regulated carbon dots (CDs). Carbon dots functionalized with amine groups (ACDs) are firstly prepared by hydrothermal treatment of citric acid and urea, then laurylamine functionalized CDs (LCDs) are obtained via the conjugation of laurylmine with ACDs. The as-prepared ACDs and LCDs provide clear and bright imaging results for lysosome and endoplasmic reticulum, respectively. The subcellular targeting features of the two CDs are attributed to their surface chemistries and cellular uptake pathways. Moreover, both the two CDs are pH responsive within certain pH range, i.e., 4.0-5.4 for ACDs and 6.2-7.2 for LCDs. The ACDs and LCDs are thus successfully applied to visualize the pH fluctuances of lysosome and endoplasmic reticulum in MCF-7 cells.

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Publication details

The article was received on 27 Apr 2018, accepted on 04 Jun 2018 and first published on 05 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR03453B
Citation: Nanoscale, 2018, Accepted Manuscript
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    Targeted imaging of lysosome and endoplasmic reticulum and their pH monitoring with surface regulated carbon dots

    S. E, Q. Mao, X. Yuan, X. Kong, X. Chen and J. Wang, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR03453B

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