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Ratiometric Photoacoustic Nanoprobe for Monitoring and Imaging of Hydrogen Sulfide in Vivo

Abstract

Detection and visualization of hydrogen sulphide (H2S) is crucial for understanding its physiological and pathological roles towards human health and disease, but precisely tracking of H2S in vivo remains challenging due to the limitations of available analytical methods. In this study, we developed a novel ratiometric photoacoustic (PA) nanoprobe for selective detection and imaging of H2S in biological fluids, live cells, brain tissues and animals. The nanoprobe AzHD-LP was fabricated by encapsulation of a newly synthesized H2S-responsive near-infrared (NIR) dye (AzHD) within a liposome (LP). The as-prepared AzHD-LP exhibits dramatically red-shift response of its absorption peak after reduction reaction of AzHD with H2S: the absorbance of AzHD-LP centered at 600 and 700 nm are decreased and increased, respectively, producing a turn-on ratiometric PA signal in the presence of H2S. Typically, under the excitation of 532-nm and 700-nm pulsed laser, the selective detection and imaging of H2S was achieved in aqueous solution, living cells and brain tissues of Aldzeme’s diseased mice. Moreover, after AzHD-LP conjugated with a tumor-targeting peptide – c(RGDyK) as RGD-AzHD-LP, ratiometric PA mapping of the intratumoral generated H2S in the HCT116 colon tumor-bearing live mice was demonstrated.

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Publication details

The article was received on 27 Apr 2018, accepted on 30 May 2018 and first published on 12 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR03445A
Citation: Nanoscale, 2018, Accepted Manuscript
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    Ratiometric Photoacoustic Nanoprobe for Monitoring and Imaging of Hydrogen Sulfide in Vivo

    T. Ma, J. Zheng, T. Zhang and D. Xing, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR03445A

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