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Issue 25, 2018
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Hydrophilic tripeptide-functionalized magnetic metal–organic frameworks for the highly efficient enrichment of N-linked glycopeptides

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Abstract

Hydrophilic interaction liquid chromatography (HILIC) is a useful tool in glycoproteomic analysis. Glutathione (GSH) is a well-known zwitterionic tripeptide with great hydrophilicity and biocompatibility and is ubiquitous in biological activities. In this study, a hydrophilic metal–organic framework (denoted as mMOF@Au@GSH) was synthesized by grafting glutathione on Au-immobilized magnetic MOFs via the affinity between the thiol group in glutathione and Au. Endowed with the high hydrophilicity of glutathione, the large surface area of the MOF and strong magnetic responsiveness of magnetic nanoparticles, the as-prepared mMOF@Au@GSH exhibited high selectivity (1 : 100) and great sensitivity (0.5 fmol μL−1) towards glycopeptides. Furthermore, it also achieved outstanding performance in enriching glycopeptides from complex biological samples. In all, 273 glycopeptides corresponding to 94 glycoproteins were identified from only 2 μL human serum.

Graphical abstract: Hydrophilic tripeptide-functionalized magnetic metal–organic frameworks for the highly efficient enrichment of N-linked glycopeptides

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Publication details

The article was received on 18 Apr 2018, accepted on 22 May 2018 and first published on 25 May 2018


Article type: Paper
DOI: 10.1039/C8NR03174F
Citation: Nanoscale, 2018,10, 12149-12155
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    Hydrophilic tripeptide-functionalized magnetic metal–organic frameworks for the highly efficient enrichment of N-linked glycopeptides

    Q. Liu, C. Deng and N. Sun, Nanoscale, 2018, 10, 12149
    DOI: 10.1039/C8NR03174F

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