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808nm light-excited upconversion nanoprobe based on LRET for the ratiometric detection of nitric oxide in living cancer cells

Abstract

NO (nitric oxide) features dual functions in cancer, promoting carcinogenesis with low concentration and inducing tumor cells apoptosis with high concentration. The dual-sword functions of NO make it particularly appealing to develop a sensitive and specific chemical probe to detect NO. However, most of NO sensors suffer from poor Stokes shift and are limited by ultraviolet (UV) or visible light excitation, which renders it difficult to avoid the intrinsic background signal. In this study, an 808nm laser-excited, Nd3+-sensitized upconversion nanoprobe based on LRET (luminescence resonance energy-transfer) for NO detection was constructed for the first time. This probe was composed of Nd3+ sensitized core-shell upconversion nanoparticles (540nm and 660nm emission), as the energy donor, and RhBs as the acceptor. At the presence of NO, RhBs was reacted into Rhodamine B and its strong absorption subsequently quenched 540nm fluorescence of UCNPs, while 660nm emission remains constant. The ratiometric of the fluorescence at 540nm over 660nm could precisely respond to the difference in NO level, with detection limit of 0.21µM. Importantly, compared with conventional UCNPs excited at 980nm, 808nm light excitation possessed lower water absorption and deeper tissue penetration depth, thus avoiding overheating and allowing for long-term biological imaging. This strategy was perfectly applied to detect the NO level in the living cells, which could differentiate the tumor cells from normal cells based on varied intracellular NO concentration. Further, the nanosystem realized real time monitoring of NO during the treatment by NO donor drugs, which inspired future application of this probe to guide NO therapy.

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Publication details

The article was received on 16 Apr 2018, accepted on 16 May 2018 and first published on 16 May 2018


Article type: Paper
DOI: 10.1039/C8NR03078B
Citation: Nanoscale, 2018, Accepted Manuscript
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    808nm light-excited upconversion nanoprobe based on LRET for the ratiometric detection of nitric oxide in living cancer cells

    H. Wang, Y. Liu, Z. Wang, M. Yang and Y. Gu, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR03078B

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