Jump to main content
Jump to site search


Target-Initiated Synthesis of Fluorescent Copper Nanoparticles for Sensitive and Label-Free Detection of Bleomycin

Abstract

Fluorescent copper nanoparticles (CuNPs) have received great attention due to their distinct characteristics of facile synthesis, tunable fluorescence emission, high photostability, and biological compatibility, and they have been widely used for chemical and biological analysis. Bleomycins (BLMs) are widely used antitumor agents for clinical treatment of various cancers. Here, we develop a sensitive and label-free fluorescent method for quantitative detection of BLM on the basis of BLM-initiated enzymatic polymerization-mediated synthesis of fluorescent CuNPs. We design two hairpin DNAs: one (Hp1) for the recognition of BLM and the other (Hp2) for signal amplification. In the presence of BLM, it may recognize and cleave the 5’-GC-3’ site of Hp1 stem, releasing the 8-17 DNAzyme fragment. The resultant 8-17 DNAzyme fragments may bind with the loop of Hp2 to form partial double-stranded DNA (dsDNA) duplex, initiating cyclic cleavage of Hp2 in the presence of Zn2+-dependent DNAzymes and generating numerous new DNA fragments with the free 3’-OH terminal, which can induce the formation of poly(thymine) (poly-T) sequence with the assistance of terminal deoxynucleotidyl transferase (TdTase). Subsequently, the ploy-T sequence may function as the template for the synthesis of CuNPs with strong fluorescence emission. This method shows good selectivity and high sensitivity with a detection limit of as low as 8.1 × 10-16 M, and it exhibits good performance in serum samples. Moreover, this method has distinct advantages of simplicity and low-cost, holding great potential in clinical diagnosis and biomedical research.

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Apr 2018, accepted on 10 May 2018 and first published on 11 May 2018


Article type: Paper
DOI: 10.1039/C8NR02780C
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    Target-Initiated Synthesis of Fluorescent Copper Nanoparticles for Sensitive and Label-Free Detection of Bleomycin

    D. Zhang, J. Hu, X. Yang, Y. Wu, W. Su and C. Zhang, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR02780C

Search articles by author

Spotlight

Advertisements