Jump to main content
Jump to site search


A Novel Alendronate Functionalized Nanoprobe for Simple Colorimetric Detection of Cancer-Associated Hypercalcemia

Abstract

Calcium (Ca2+) ion concentration in the blood serum is tightly regulated, and any abnormalities in the level of serum calcium ion are associated with many potentially dangerous diseases. Thus, monitoring of Ca2+ ion concentration in the blood serum is of fundamental importance. Gold nanoparticle (GNP)-based colorimetric biosensors have enormous potential in clinical diagnostic applications due to their simplicity, versatility, and unique optical properties. In this study, we have developed alendronate functionalized gold nanoparticle (GNP-ALD) system for the measurement of Ca2+ ion concentration in biological samples. GNP-ALD system showed higher sensitivity towards the Ca2+ ion compared to adenosine diphosphate (ADP) or adenosine triphosphate (ATP). The strong interaction between Ca2+ ion and ALD at the GNP/solution interface resulted in significant aggregation of the ALD conjugated GNPs, and induced a color change of the solution from red to blue which could be visually observed by the naked eye. The interaction between Ca2+ ion and GNP-ALD was characterized by UV-visible spectroscopy, transmission electron microscopy (TEM) imaging, and dynamic light scattering (DLS) analysis. Under the optimized condition, the lower limit of Ca2+ ion detection using this method was found to be 25 µM and a linear response range from 25 µM to 300 µM Ca2+ ion was obtained with excellent discrimination against other metal ions. The GNP-ALD nanoprobe could successfully determine the ionized Ca2+ concentration in various serum samples and the results were validated by a commercial calcium assay kit. Moreover, as a practical application, we demonstrated the utility of this nanoprobe for the detection of cancer-associated hypercalcemia in a mouse model.

Back to tab navigation

Supplementary files

Publication details

The article was accepted on 13 Apr 2018 and first published on 27 Apr 2018


Article type: Paper
DOI: 10.1039/C8NR02570C
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    A Novel Alendronate Functionalized Nanoprobe for Simple Colorimetric Detection of Cancer-Associated Hypercalcemia

    A. Sahu, Y. Hwang, C. Vilos, J. Lim, S. Kim, W. I. Choi and G. Tae, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR02570C

Search articles by author

Spotlight

Advertisements