Jump to main content
Jump to site search


Phosphorescent Inner Filter Effect-based Sensing of Xanthine Oxidase and its Inhibitors with Mn-doped ZnS Quantum Dots

Abstract

Uric acid overexpress and crystallization has been recognized as the course of hyperuricemia and gout, which is produced via xanthine oxidase (XOD)-catalyzed oxidation of xanthine. Therefore, medicinal therapy of hyperuricemia and gout is majorly based on the inhibition of XOD enzymatic pathway. The spectroscopic nature of xanthine and uric acid, namely both absorption (near ultraviolet region) and emission (non-fluorescent) characteristics, hinders optical assay development for XOD analysis. Therefore, the state-of-the-art analysis of XOD and screening of XOD inhibitors are majorly based on chromatography. Here, we found the near ultraviolet absorption of uric acid overlapped well with the absorption of a large bandgap semiconductor quantum dots, ZnS. On the other hand, the intrinsic weak fluorescence of ZnS QDs can be substantially improved via transition metal ion doping. Therefore, herein we developed an inner filter effect-based assay for XOD analysis and inhibitor screening with Mn-doped ZnS QDs. The phosphorescence of Mn-doped ZnS QDs could be quenched by uric acid generated from xanthine catabolism by XOD, leading to phosphorescence turn-off detection of XOD with a limit of detection (3σ) of 0.02 U/L. Furthermore, the existence of XOD inhibitors could inhibit the XOD enzymatic reaction, resulting in weakened phosphorescence quenching. Therefore, the proposed assay could also be explored for facile screening analysis of XOD inhibitors, which is important for potential medicinal therapy of hyperuricemia and gout.

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Feb 2018, accepted on 02 Apr 2018 and first published on 02 Apr 2018


Article type: Paper
DOI: 10.1039/C8NR01355A
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    Phosphorescent Inner Filter Effect-based Sensing of Xanthine Oxidase and its Inhibitors with Mn-doped ZnS Quantum Dots

    D. Tang, J. Zhang, R. Zhou, Y. Xie, X. Hou, K. Xu and P. Wu, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR01355A

Search articles by author

Spotlight

Advertisements