Jump to main content
Jump to site search


Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis

Abstract

Detection and counting of single virus particle in liquid samples is largely limited to narrow size distribution viruses and purified formulations. To address these limitations here we propose a calibration-free method that enables concurrently the selective recognition, counting and sizing of virus particles as demonstrated through the detection of human respiratory syncytial virus (RSV), an enveloped virus with broad size distribution, in throat swab samples. RSV viruses were selectively labeled through their attachment glycoproteins (G) with fluorescent aptamers, which further enabled their identification, sizing and counting at single particle level by fluorescent nanoparticle tracking analysis. The proposed approach seems to be generally applicable to virus detection and quantification. Moreover, it could be successfully applied to detect single RSV particles in diluted throat swab samples of diagnostic relevance. Since the selective recognition is associated with the sizing of each detected particle the method enables to discriminate between viral elements linked to the virus as well as between various virus forms and associations.

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Feb 2018, accepted on 26 Apr 2018 and first published on 26 Apr 2018


Article type: Paper
DOI: 10.1039/C8NR01310A
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis

    Z. Szakács, T. Mészáros, M. I. De Jonge and R. E. Gyurcsányi, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR01310A

Search articles by author

Spotlight

Advertisements