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The Owl Sensor: a ‘Fragile’ DNA Nanostructure for Analysis of Single Nucleotide Variations

Abstract

Analysis of single nucleotide variations (SNVs) in DNA and RNA sequences is instrumental in healthcare for detection of genetic and infectious diseases and drug-resistant pathogens. Here we took advantage of developments in DNA nanotechnology to design a hybridization sensor, named the ‘Owl Sensors’, which produce a fluorescent signal only in complex with a fully complementary DNA or RNA analytes. The nov-elty of Owl Sensor operation is that selectivity of analyte recognition is, at least in part, determined by the structural rigidity and stability of the entire DNA nanostructure rather than exclusively by the stability of the analyte-probe duplex, as is the case for conventional hybridization probes. Using two DNA and two RNA analytes we demonstrated that Owl Sensors differentiate SNVs in a wide temperature range of 5°C-32°C, a performance unachievable by conventional hybridization probes including molecular beacon probe. Owl Sensor’s reliably detects cognate analyes even in the presence of 100 times excess of single base mismatched sequences. The approach, therefore, promises to add to the toolbox for diagnosis of SNVs at ambient temperatures.

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Publication details

The article was received on 07 Feb 2018, accepted on 26 Apr 2018 and first published on 26 Apr 2018


Article type: Paper
DOI: 10.1039/C8NR01107A
Citation: Nanoscale, 2018, Accepted Manuscript
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    The Owl Sensor: a ‘Fragile’ DNA Nanostructure for Analysis of Single Nucleotide Variations

    R. J. Karadeema, M. Stancescu, T. P. Steidl, S. C. Bertot and D. M. Kolpashchikov, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR01107A

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