Jump to main content
Jump to site search

Issue 15, 2018
Previous Article Next Article

A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells

Author affiliations

Abstract

The design of a nano-system for the detection of intracellular microRNAs is challenging as it must fulfill complex requirements, i.e., it must have a high sensitivity to determine the dynamic expression level, a good reliability for multiplex and simultaneous detection, and a satisfactory biostability to work in biological environments. Instead of employing a commonly used physisorption or a full-conjugation strategy, here, a GO-DNA nano-system was developed under graft/base-pairing construction. The common anchor sequence was chemically grafted to GO to base-pair with various microRNA probes; and the hybridization with miRNAs drives the dyes on the probes to leave away from GO, resulting in “turned-on” fluorescence. This strategy not only simplifies the synthesis but also efficiently balances the loading yields of different probes. Moreover, the conjugation yield of GO with a base-paired hybrid has been improved by more than two-fold compared to that of the conjugation with a single strand. We demonstrated that base-paired DNA probes could be efficiently delivered into cells along with GO and are properly stabilized by the conjugated anchor sequence. The resultant GO-DNA nano-system exhibited high stability in a complex biological environment and good resistance to nucleases, and was able to accurately discriminate various miRNAs without cross-reaction. With all of these positive features, the GO-DNA nano-system can simultaneously detect three miRNAs and monitor their dynamic expression levels.

Graphical abstract: A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Jan 2018, accepted on 12 Mar 2018 and first published on 13 Mar 2018


Article type: Paper
DOI: 10.1039/C8NR00364E
Citation: Nanoscale, 2018,10, 7067-7076
  •   Request permissions

    A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells

    J. Yu, S. He, C. Shao, H. Zhao, J. Li and L. Tian, Nanoscale, 2018, 10, 7067
    DOI: 10.1039/C8NR00364E

Search articles by author

Spotlight

Advertisements