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Common Anchor Facilitated GO-DNA Nano-system for Multiplex MicroRNA Analysis in Live Cells


The design of a nano-system for the detection of intracellular microRNAs is challenging as it must fulfill complex requirements, i.e., it must have a high sensitivity to determine the dynamic expression level, a good reliability for multiplex and simultaneous detection, and a satisfactory biostability to work in biological environments. Instead of employing a commonly used a physisorption or a full-conjugation strategy, here, a GO-DNA nano-system was developed under graft/base-pairing construction. The common anchor sequence was chemically grafted to GO to base-pair with various microRNA probes; and the hybridization with miRNAs drives the dyes on the probes to leave away from GO, resulting in “turned-on” fluorescence. This strategy not only simplifies the synthesis but also efficiently balances the loading yields of different probes. Moreover, the conjugation yield for GO with a base-paired hybrid has been improved by more than two-fold compared that of the conjugation with a single strand. We demonstrated that base-paired DNA probes could be efficiently delivered into cells along with GO and are properly stabilized by the conjugated anchor sequence. The resultant GO-DNA nano-system exhibited high stability in a complex biological environment and good resistance to nucleases, and was able to accurately discriminate various miRNAs without cross-reaction. With all of these positive features, the GO-DNA nano-system can simultaneously detect three miRNAs and monitor their dynamic expression levels.

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Publication details

The article was received on 14 Jan 2018, accepted on 12 Mar 2018 and first published on 13 Mar 2018

Article type: Paper
DOI: 10.1039/C8NR00364E
Citation: Nanoscale, 2018, Accepted Manuscript
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    Common Anchor Facilitated GO-DNA Nano-system for Multiplex MicroRNA Analysis in Live Cells

    J. Yu, S. He, S. Chen, H. Zhao, J. Li and L. Tian, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR00364E

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