Jump to main content
Jump to site search


HIV-related DNA detection through switching on hybridized quenched fluorescent DNA-Ag nanocluster

Abstract

In this paper, DNA containing six cytosines as formation site for Ag NC was adopted as template for preparing fluorescent DNA-Ag NC. It was the first time to find fluorescence of DNA-Ag NC could be quenched after hybridization with its complementary sequence. On the basis of this new phenomenon, we designed a sequence C1 that was completely complementary matching to human immunodeficiency virus (HIV) DNA, and probe DNA which was partially complementary to C1 for synthesis of DNA-Ag NC. The fluorescence of DNA-Ag NC was quenched after hybridization with C1 and formed DNA-Ag NC/C1 composite, while C1 could be dissociated away from the DNA-Ag NC by HIV DNA through strand exchange reaction due to the stronger affinity between HIV DNA and C1, which could switch on the quenched Ag NC, thus a new “off-on” fluorescence method for HIV detection was fabricated. In experiment, the Ag NC formation site of DNA, the number of base pairs, pH and salt concentration of binding buffer were optimized. Under the optimum conditions, the limit of detection for HIV DNA was obtained to be 3.18 nM (3σ/N, n = 7) with the linear range of 15 – 150 nM for 150 nM DNA-Ag NC/C1 probe. Besides, the probe showed excellent specificity to HIV DNA, even distinguished one nucleotide mismatched HIV DNA.

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Dec 2017, accepted on 08 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C7NR09647J
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    HIV-related DNA detection through switching on hybridized quenched fluorescent DNA-Ag nanocluster

    B. Fang, C. Li, J. An, S. Zhao, Z. Zhuang, Y. Zhao and Y. Zhang, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR09647J

Search articles by author

Spotlight

Advertisements