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Free-Electron-Laser Coherent Diffraction Images Individual Drug-Carrying Liposome Particles in Solution

Abstract

Using the excellent performances of the SACLA (RIKEN/HARIMA, Japan) X-ray free electron laser (X-FEL), coherent diffraction imaging (CDI) was used to detect individual liposome particles in water, with or without inserted doxorubicin nanorods. This was possible because of the electron density differences between the carrier, the liposome, and the drug. The result is important since liposome nanocarriers dominate at present the drug delivery systems. In spite of the low cross section of the original ingredients, the diffracted intensity of drug-free liposomes was sufficient for spatial reconstruction yielding quantitative structural information. For particles containing doxorubicin, the structural parameters of the nanorods could be extracted from CDI. Furthermore, the measurement of the electron density of the solution enclosed in each liposome provides direct evidence of the incorporation of ammonium sulphate into the nanorods. Overall, ours is an important test for extending the X-FEL analysis of individual nanoparticles to low cross-section-systems in solution, and also for its potential use to optimize the manufacturing of drug nanocarriers.

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Publication details

The article was accepted on 01 Jan 2018 and first published on 02 Jan 2018


Article type: Paper
DOI: 10.1039/C7NR09395K
Citation: Nanoscale, 2018, Accepted Manuscript
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    Free-Electron-Laser Coherent Diffraction Images Individual Drug-Carrying Liposome Particles in Solution

    C. Huang, K. S. Liang, T. Hsu, T. Lee, Y. Chen, S. Yang, H. Chen, S. Huang, W. Chang, P. Chen, T. Lee, K. Peng, C. Chen, C. Shi, Y. Hu, G. Margaritondo, T. Ishikawa, C. Wong and Y. Hwu, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR09395K

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