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Screening and multiple detection of cancerous exosomes using a SERS-based method

Abstract

As one kind of the most important cancerous biomarkers, exosomes are getting more frequently investigated in cancer diagnosis. In this study, we proposed a SERS-based method for the screening and simultaneous multiple detection of exosomes using magnetic substrates and SERS probes. Specifically, the capturing substrates are achieved by the gold shell magnetic nanobeads modified by aptamers, which can collect most kinds of exosomes by recognizing the generic surface protein of CD63. Moreover, the SERS probes are made of gold nanoparticles decorated with a Raman reporter and a specific aptamer for targeting exosomes. Further, for the simultaneous detection of multiple kinds of exosomes, three kinds of SERS probes were designed using different SERS reporters. When detecting the specific kind of exosomes, the capturing substrates were mixed with three kinds of SERS probes. With the target exosome present, an apta-immunocomplex can be formed among the target exosomes, the substrate and the corresponding kind of SERS probes, leaving the other non-specific SERS probes in the suspension. Hence, the SERS signal with a decreased intensity will be detected in the supernatant, indicating the presence of the target exosomes. Finally, such a detection method has also successfully been employed for detecting the exosomes in real blood samples, which proves that the presented SERS-based method is a promising tool for the clinical cancer screening based on exosomes.

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Publication details

The article was received on 09 Dec 2017, accepted on 27 Mar 2018 and first published on 09 Apr 2018


Article type: Paper
DOI: 10.1039/C7NR09162A
Citation: Nanoscale, 2018, Accepted Manuscript
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    Screening and multiple detection of cancerous exosomes using a SERS-based method

    Z. Wang, S. Zong, Y. Wang, N. Li, L. Li, J. Lu, Z. Wang, B. Chen and Y. Cui, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR09162A

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