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Study of Diffusion Dynamics and Concentration Distribution of Gold Nanospheres (GNSs) without Fluorescent Labeling inside Live Cells Using Fluorescence Single Particle Spectroscopy

Abstract

Colloidal gold nanospheres (GNSs) have become important nanomaterials in biomedical applications due to their special optical properties, good chemical stability, and biocompatibility. However, how to in situ measure the diffusion coefficients or concentration distribution of GNSs within live cells accurately, cannot still be resolved without any extra fluorescent labeling. In this work, a single particle method is developed to study the concentration distribution of folic acid-modified GNSs (FA-GNSs) internalized via folate receptors, and investigates their diffusion dynamics within live cells using single particle fluorescence correlation spectroscopy (FCS). We optimized the experimental conditions and verified the feasibility of 30 nm GNSs without extra fluorescent labeling used for single particle detection insides live cells. And then, the FCS characterization strategy was used to measure the concentration and diffusion coefficient distributions of GNSs inside live cells and the obtained results are basically in agreement with that by TEM. The results demonstrate that our strategy is characterized as in situ, nondestructuctive, rapid and dynamic method for assay of live cells, and it may be widely used in the further design of GNP-based drug delivery and therapeutics.

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Supplementary files

Publication details

The article was received on 22 Nov 2017, accepted on 08 Feb 2018 and first published on 08 Feb 2018


Article type: Paper
DOI: 10.1039/C7NR08722E
Citation: Nanoscale, 2018, Accepted Manuscript
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    Study of Diffusion Dynamics and Concentration Distribution of Gold Nanospheres (GNSs) without Fluorescent Labeling inside Live Cells Using Fluorescence Single Particle Spectroscopy

    F. Liu, C. Dong and J. Ren, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR08722E

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