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Stemmed DNA nanostructure for the selective delivery of therapeutics

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Abstract

DNA has emerged as a biocompatible biomaterial that may be considered for various applications. Here, we report tumor cell-specific aptamer-modified DNA nanostructures for the specific recognition and delivery of therapeutic chemicals to cancer cells. Protein tyrosine kinase (PTK)7-specific DNA aptamer sequences were linked to 15 consecutive guanines. The resulting aptamer-modified product, AptG15, self-assembled into a Y-shaped structure. The presence of a G-quadruplex at AptG15 was confirmed by circular dichroism and Raman spectroscopy. The utility of AptG15 as a nanocarrier of therapeutics was tested by loading the photosensitizer, methylene blue (MB), to the G-quadruplex as a model drug. The generated MB-loaded AptG15 (MB/AptG15) showed specific and enhanced uptake to CCRF-CEM cells, which overexpress PTK7, compared with Ramos cells, which lack PTK7, or CCRF-CEM cells treated with a PTK7-specific siRNA. The therapeutic activity of MB/AptG15 was tested by triggering its photodynamic effects. Upon 660 nm light irradiation, MB/AptG15 showed greater reactive oxygen species generation and anticancer activity in PTK7-overexpressing cells compared to cells treated with MB alone, those treated with AptG15, and other comparison groups. AptG15 stemmed DNA nanostructures have significant potential for the cell-type-specific delivery of therapeutics, and possibly for the molecular imaging of target cells.

Graphical abstract: Stemmed DNA nanostructure for the selective delivery of therapeutics

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Publication details

The article was received on 16 Nov 2017, accepted on 23 Feb 2018 and first published on 02 Mar 2018


Article type: Paper
DOI: 10.1039/C7NR08558C
Citation: Nanoscale, 2018, Advance Article
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    Stemmed DNA nanostructure for the selective delivery of therapeutics

    H. Jin, M. G. Kim, S. B. Ko, D. H. Kim, B. J. Lee, R. B. Macgregor, Jr., G. Shim and Y. K. Oh, Nanoscale, 2018, Advance Article , DOI: 10.1039/C7NR08558C

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