Jump to main content
Jump to site search

Issue 11, 2018
Previous Article Next Article

Neuronal adhesion and growth on nanopatterned EA5-POPC synthetic membranes

Author affiliations

Abstract

Biomimetic membranes create opportunities for various applications, including the possibility of replacing interacting cells in a cell–cell contact. Here we have fractionated synthetic membranes using metal nano-grid structures where EphrinA5 (EA5), a neuronal adhesion promoter, was anchored via its Fc domain (immunoglobulin G (IgG)-domain). FRAP experiments were performed to check the confinement of the synthetic membrane within these nano-structures. Rat cortical primary neurons were cultured and live cell imaging techniques were used to monitor the neuronal interaction with these fractionated synthetic membranes. Computational imaging analysis of the corresponding images elucidated interesting details of the cellular behavior. The phenotypic cellular response on these nano-membrane fractions was found to be similar to that on non-fractionated synthetic membranes indicating that although the number of focal adhesion points was low (due to the reduced EA5 number) in the nano-sized membrane patches perhaps some other factors like metal grid boundaries might be playing a role in rendering the similarity.

Graphical abstract: Neuronal adhesion and growth on nanopatterned EA5-POPC synthetic membranes

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Nov 2017, accepted on 18 Feb 2018 and first published on 20 Feb 2018


Article type: Paper
DOI: 10.1039/C7NR08520F
Citation: Nanoscale, 2018,10, 5295-5301
  •   Request permissions

    Neuronal adhesion and growth on nanopatterned EA5-POPC synthetic membranes

    R. Ghosh Moulick, G. Panaitov, L. Du, D. Mayer and A. Offenhäusser, Nanoscale, 2018, 10, 5295
    DOI: 10.1039/C7NR08520F

Search articles by author

Spotlight

Advertisements