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Issue 3, 2018
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Technical challenges of working with extracellular vesicles

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Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Graphical abstract: Technical challenges of working with extracellular vesicles

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Publication details

The article was received on 09 Nov 2017, accepted on 08 Dec 2017 and first published on 13 Dec 2017


Article type: Review Article
DOI: 10.1039/C7NR08360B
Citation: Nanoscale, 2018,10, 881-906
  • Open access: Creative Commons BY license
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    Technical challenges of working with extracellular vesicles

    M. I. Ramirez, M. G. Amorim, C. Gadelha, I. Milic, J. A. Welsh, V. M. Freitas, M. Nawaz, N. Akbar, Y. Couch, L. Makin, F. Cooke, A. L. Vettore, P. X. Batista, R. Freezor, J. A. Pezuk, L. Rosa-Fernandes, A. C. O. Carreira, A. Devitt, L. Jacobs, I. T. Silva, G. Coakley, D. N. Nunes, D. Carter, G. Palmisano and E. Dias-Neto, Nanoscale, 2018, 10, 881
    DOI: 10.1039/C7NR08360B

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