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Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

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Abstract

Direct cellular delivery of CRISPR/Cas9 complexes is of great significance for genome editing and other recently developed applications, such as gene expression regulation and RNA/DNA imaging. Here, we first constructed a graphene oxide (GO)-polyethylene glycol (PEG)-polyethylenimine (PEI) nanocarrier for the delivery of high-molecular-weight Cas9/single-guide RNA (sgRNA) complexes for endocytosis, endosomal escape, nuclear entry, and gene editing. The results demonstrate that the nanocarrier can be used successfully for efficient gene editing in human AGS cells with an efficiency of ∼39%. The results also show that this nanocarrier can protect sgRNA from enzymatic degradation, thus exhibiting extremely high stability, which is critical for future in vivo applications. Thus, this GO-mediated Cas9/sgRNA delivery system has potential as a new approach for biomedical research and targeted gene engineering applications.

Graphical abstract: Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

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Publication details

The article was received on 27 Oct 2017, accepted on 17 Dec 2017 and first published on 18 Dec 2017


Article type: Paper
DOI: 10.1039/C7NR07999K
Citation: Nanoscale, 2018, Advance Article
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    Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

    H. Yue, X. Zhou, M. Cheng and D. Xing, Nanoscale, 2018, Advance Article , DOI: 10.1039/C7NR07999K

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