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Self-Standing Aptamers by Artificial Defect-Rich Matrix

Abstract

The classical alkanethiol post-passivation can prevent nucleotide bases nonspecific binding onto supporting substrates and help aptamers from “lying down” to “standing up” orientation. However, the surface probes represent lower binding affinity toward targets than those in bulk solutions due to unsatisfied hybridization spaces on the alkanethiol passivated substrate. To overcome this challenge, an artificial defect-rich matrix possessing aptamer “self-standing” property created by chemical lift-off lithography (CLL) is demonstrated. This approach provides artificial defects on hydroxyl-terminated alkanethiol self-assembled monolayer (SAM), which allows the insertion of thiolated aptamers. The diluted surface molecular environment assists aptamers not only to “self-stand” on the surface but also to be separated from each other, providing suitable surface aptamer density and sufficient space for capturing targets. The binding affinity of aptamer toward target in this approach is found to be comparable to solution-type probes, showing higher recognition efficiency than that in conventional methods.

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Publication details

The article was received on 04 Oct 2017, accepted on 12 Jan 2018 and first published on 12 Jan 2018


Article type: Paper
DOI: 10.1039/C7NR07381J
Citation: Nanoscale, 2018, Accepted Manuscript
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    Self-Standing Aptamers by Artificial Defect-Rich Matrix

    C. Chen, C. Wang, P. Chen and W. Liao, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR07381J

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