Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Tuesday 6th February 2018 from 11.00am to 11.15am (GMT).

During this time our website performance may be temporarily affected. If you have any questions please use the feedback button available under our menu button. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 3, 2018
Previous Article Next Article

Probing fibronectin conformation on a protein corona layer around nanoparticles

Author affiliations

Abstract

Protein unfolding induced by nanoparticles (NPs) can lead to exposure of cryptic epitopes that might dictate biological identity and affect NP biological fate (e.g., blood circulation time, biodistribution, and tumor accumulation). Here, we monitor the conformation of fluorescence resonance energy transfer (FRET)-labelled fibronectin (FN) on corona-coated gold NPs. We found that the labelled FN proteins, which directly accessed the gold NP surface, underwent more pronounced conformational changes than those associated with the protein corona via protein–protein interactions. FRET and liquid chromatography–mass spectrometry analyses demonstrated that NP size/concentration, pH change, and the level of surface coverage by the corona can tune the accessibility of labelled FN to the gold NP surface. Although some subsequently adsorbing proteins accessed the NP surface thanks to incomplete surface coverage and protein exchange (the Vroman effect), most outer-layer proteins could not directly bind to the NP surface, blocked by pre-adsorbed corona layers. This finding was also partially confirmed by isothermal titration calorimetry (ITC) analysis. These results suggest the proof-of-concept that outermost-layer proteins with modestly changed conformation rather than unfolded proteins at the gold NP surface effectively create the NPs’ biological identity, which might have important implications on biological fates of gold NPs.

Graphical abstract: Probing fibronectin conformation on a protein corona layer around nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Sep 2017, accepted on 08 Dec 2017 and first published on 11 Dec 2017


Article type: Paper
DOI: 10.1039/C7NR06970G
Citation: Nanoscale, 2018,10, 1228-1233
  •   Request permissions

    Probing fibronectin conformation on a protein corona layer around nanoparticles

    M. Raoufi, M. J. Hajipour, S. M. Kamali Shahri, I. Schoen, U. Linn and M. Mahmoudi, Nanoscale, 2018, 10, 1228
    DOI: 10.1039/C7NR06970G

Search articles by author

Spotlight

Advertisements