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Citrate stabilized Gold Nanoparticles interfere with Amyloid Fibril formation: D76N and ∆N6 β2-microglobulin Variants

Abstract

Protein aggregation including the formation of dimers and multimers in solution, underlies an array of human diseases such as systemic amyloidosis which is a fatal disease caused by misfolding of native globular proteins damaging the structure and function of affected organs. Different kind of interactors can interfere with the formation of protein dimers and multimers in solution. A very special class of interactors are nanoparticles thanks to the extremely efficient extension of their interaction surface. In particular citrate-coated gold nanoparticles (cit-AuNPs) were recently addressed with amyloidogenic protein β2-microglobulin (b2m). Here we present the computational studies on two challenging models known for their en- hanced amyloidogenic propensity, namely ∆N6 and D76N b2m naturally occuring variants, and disclosing the role of cit-AuNPs on their fibrillogenesis. The proposed interaction mech- anism lies in the interference of the cit-AuNPs with the protein dimers at the early stages of aggregation, that induces dimer disassembling. As a consequence, natural fibril formation can be inhibited. Relying on the comparison between atomistic simulations at multiple levels (enhanced sam- pling molecular dynamics and Brownian dynamics) and protein structural characterization by NMR, we demonstrate that the cit-AuNPs interactors are able to inhibit protein dimer assembling. As a consequence, the natural fibril formation is also inhibited, as found in experiment.

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Publication details

The article was received on 12 Sep 2017, accepted on 13 Feb 2018 and first published on 14 Feb 2018


Article type: Paper
DOI: 10.1039/C7NR06808E
Citation: Nanoscale, 2018, Accepted Manuscript
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    Citrate stabilized Gold Nanoparticles interfere with Amyloid Fibril formation: D76N and ∆N6 β2-microglobulin Variants

    G. Brancolini, C. Cantarutti, A. Corazza, F. Fogolari, S. Corni, G. Esposito, M. C. Maschio and V. Bellotti, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C7NR06808E

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