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Issue 14, 2018
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Construction of artificial cilia from microtubules and kinesins through a well-designed bottom-up approach

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Abstract

Self-organized structures of biomolecular motor systems, such as cilia and flagella, play key roles in the dynamic processes of living organisms, like locomotion or the transportation of materials. Although fabrication of such self-organized structures from reconstructed biomolecular motor systems has attracted much attention in recent years, a systematic construction methodology is still lacking. In this work, through a bottom-up approach, we fabricated artificial cilia from a reconstructed biomolecular motor system, microtubule/kinesin. The artificial cilia exhibited a beating motion upon the consumption, by the kinesins, of the chemical energy obtained from the hydrolysis of adenosine triphosphate (ATP). Several design parameters, such as the length of the microtubules, the density of the kinesins along the microtubules, the depletion force among the microtubules, etc., have been identified, which permit tuning of the beating frequency of the artificial cilia. The beating frequency of the artificial cilia increases upon increasing the length of the microtubules, but declines for the much longer microtubules. A high density of the kinesins along the microtubules is favorable for the beating motion of the cilia. The depletion force induced bundling of the microtubules accelerated the beating motion of the artificial cilia and increased the beating frequency. This work helps understand the role of self-assembled structures of the biomolecular motor systems in the dynamics of living organisms and is expected to expedite the development of artificial nanomachines, in which the biomolecular motors may serve as actuators.

Graphical abstract: Construction of artificial cilia from microtubules and kinesins through a well-designed bottom-up approach

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Publication details

The article was received on 13 Jul 2017, accepted on 22 Feb 2018 and first published on 23 Feb 2018


Article type: Paper
DOI: 10.1039/C7NR05099B
Citation: Nanoscale, 2018,10, 6323-6332
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    Construction of artificial cilia from microtubules and kinesins through a well-designed bottom-up approach

    R. Sasaki, A. Md. R. Kabir, D. Inoue, S. Anan, A. P. Kimura, A. Konagaya, K. Sada and A. Kakugo, Nanoscale, 2018, 10, 6323
    DOI: 10.1039/C7NR05099B

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