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Issue 14, 2018
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A highly sensitive fluorescent probe for bioimaging zinc ion in living cells and zebrafish models

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Abstract

Zn2+ plays fundamental roles in cellular metabolism and apoptosis, as well as neurotransmission. However, how to fast detect Zn2+ in biology is still challenging and meaningful. In this study, we rationally synthesized a simple OFF–ON fluorescent probe for fast detection of Zn2+ based on intramolecular charge transfer and chelation-enhanced fluorescence. Addition of Zn2+ into the probe induced a significant fluorescence enhancement with a bathochromic shift of 55 nm. Moreover, the probe demonstrated excellent sensitivity (detection limit = 0.072 μM) and outstanding selectivity toward Zn2+ and an ultra-fast response rate of two seconds. The recognition mechanism of the probe toward Zn2+ was fully confirmed by HRMS and 1H NMR spectra, as well as TDDFT calculations. Furthermore, the probe could rapidly visualize Zn2+ in living cells and zebrafish models in bioimaging experiments with low cytotoxicity and good biocompatibility.

Graphical abstract: A highly sensitive fluorescent probe for bioimaging zinc ion in living cells and zebrafish models

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Publication details

The article was received on 05 May 2018, accepted on 13 Jun 2018 and first published on 13 Jun 2018


Article type: Paper
DOI: 10.1039/C8NJ02197J
Citation: New J. Chem., 2018,42, 12198-12204
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    A highly sensitive fluorescent probe for bioimaging zinc ion in living cells and zebrafish models

    Z. Lu, W. Fan, Y. Lu, C. Fan, H. Zhao, K. Guo, W. Chu and Y. Lu, New J. Chem., 2018, 42, 12198
    DOI: 10.1039/C8NJ02197J

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