Jump to main content
Jump to site search


A novel dual-emission fluorescent nanohybrid containing silica nanoparticles and gold nanoclusters for ratiometric determination of cysteine based on turn-on fluorescence strategy

Abstract

The present work has been developed a novel ratiometric fluorescence sensor for detection of cysteine (Cys) by linking the recognition indicators glutathione capped gold nanoclusters (GSH-AuNCs) onto the surface of amino modified SiO2NPs@SiO2 spheres. The as-prepared nanohybrid (SiO2NPs/AuNCs) exhibits dual-emission fluorescence peaking at 425 and 605 nm under single excitation of 360 nm. Further, its morphology, composition, surface chemical features and optical properties have been fully characterized through transmission electron microscopy, x-ray photoelectron spectroscopy, Fourier transform infrared, UV-vis absorption and fluorescence spectroscopy. As for the Cys detection, the red fluorescence intensity of AuNCs of the nanohybrid can be selectively and effectively enhanced by Cys due to specific interaction between the indicator and target, while reference signal of blue emitting SiO2NPs@SiO2 spheres keep constant as the SiO2NPs are entirely encapsulated by silica shell. Thus the variation of intensity ratio (I605/I425) of nanohybrid can be used to determine Cys based on ratiometric method. The I605/I425 against the concentration of Cys demonstrates a good linear relationship from 0 to 20 M and the detection limit reaches as low as 0.35 M. Besides, this ratiometric system has been applied to determine Cys in serum samples with satisfactory results.

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Mar 2018, accepted on 06 May 2018 and first published on 11 May 2018


Article type: Paper
DOI: 10.1039/C8NJ01528G
Citation: New J. Chem., 2018, Accepted Manuscript
  •   Request permissions

    A novel dual-emission fluorescent nanohybrid containing silica nanoparticles and gold nanoclusters for ratiometric determination of cysteine based on turn-on fluorescence strategy

    N. Xu, Y. Yuan, C. Lan, W. Wei, L. Meng and L. Fan, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ01528G

Search articles by author

Spotlight

Advertisements