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Issue 11, 2018
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Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

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Abstract

Phosphate plays a central role in the environment and in biology. This has led to the demand for a specific phosphate receptor that can be used to sense, enrich or separate phosphate and phosphorylated molecules from complex mixtures. Here, we report an approach for the production of phospho-affinity porous beads with controllable size and pore structure leading to significantly improved chromatographic properties. The beads were prepared by polymerization of bis-imidazolium based host monomer and crosslinker inside the pores of macroporous silica beads that post-etching resulted in mesoporous polymer replicas. The silica precursor, silica–polymer composite and porous polymer replica were characterized by using SEM, IR spectroscopy, optical microscopy and thermogravimetric analysis. Bis-imidazolium functionalized materials were capable of binding and enriching the signaling lipid sphingosine-1-phosphate from plasma samples. Moreover, these engineered materials combined with artificial receptors can recognize phosphorylated amino acids/peptides and are applicable to selective recognition of a broad class of phosphorylated biomolecules.

Graphical abstract: Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

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Publication details

The article was received on 07 Mar 2018, accepted on 31 Mar 2018 and first published on 02 Apr 2018


Article type: Paper
DOI: 10.1039/C8NJ01114A
Citation: New J. Chem., 2018,42, 8603-8608
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    Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

    S. Shinde, J. Selvalatchmanan, A. Incel, M. Akhoundian, A. K. Bendt and F. Torta, New J. Chem., 2018, 42, 8603
    DOI: 10.1039/C8NJ01114A

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