Jump to main content
Jump to site search

Issue 13, 2018
Previous Article Next Article

A fluorescent dual aptasensor for the rapid and sensitive onsite detection of E. coli O157:H7 and its validation in various food matrices

Author affiliations

Abstract

The speedy analysis of food products remains a keen area of concern; thus, rapid, highly efficient and robust on-site detection platforms are essential. Globally, Escherichia coli O157:H7 is the most common organism associated with food borne illness. The present study focuses on the development of a rapid onsite E. coli O157:H7 detection platform. Accordingly, target specific ssDNA aptamers are generated using whole cell-SELEX, where the highly reactive aptamers (EcoR1 and EcoR2) with the lowest dissociation constants (Kd) are further biotin tagged (EcoR1) and fluorescent labelled (EcoR2). Subsequently, the biotin-labelled aptamers are immobilized onto silane-glutaraldehyde-functionalized glass slides, which act as capturing ligands, and the QD-labelled aptamers act as revealing probes. The detection limit of the platform is found to be 102 CFU mL−1 within 15 min, and no cross-reactivity towards other related pathogens is observed. The developed assay is further evaluated in complex food matrices to determine the presence of E. coli O157:H7, where the results are found to be consistent with those of the conventional methods with a recovery percentage ranging from 76% to 91%. In conclusion, the developed method is promising for application in real-time food sample monitoring.

Graphical abstract: A fluorescent dual aptasensor for the rapid and sensitive onsite detection of E. coli O157:H7 and its validation in various food matrices

Back to tab navigation

Publication details

The article was received on 01 Mar 2018, accepted on 14 May 2018 and first published on 15 May 2018


Article type: Paper
DOI: 10.1039/C8NJ00997J
Citation: New J. Chem., 2018,42, 10807-10817
  •   Request permissions

    A fluorescent dual aptasensor for the rapid and sensitive onsite detection of E. coli O157:H7 and its validation in various food matrices

    R. M. Renuka, J. Achuth, H. R. Chandan, M. Venkataramana and K. Kadirvelu, New J. Chem., 2018, 42, 10807
    DOI: 10.1039/C8NJ00997J

Search articles by author

Spotlight

Advertisements