Jump to main content
Jump to site search

Issue 7, 2018
Previous Article Next Article

Phytochemical characterization, in vitro and in silico approaches for three Hypericum species

Author affiliations

Abstract

Members of the Hypericum genus are spread throughout the world and have a long history of use in traditional systems of medicines. In this present investigation, the methanolic extracts of aerial parts of three Hypericum species (H. olympicum (HO), H. pruinatum (HP), and H. scabrum (HS)) growing in Turkey were investigated for their key enzyme inhibitory potential. The chemical profiles were evaluated using HPLC-ESI-MSn, and their total phenolic and flavonoid contents were established. Also, relevant bioactive compounds observed in the extracts were subjected to molecular docking experiments to provide insights into their interactions with the tested enzymes. HPLC-ESI-MSn analysis revealed that the extract of HO, followed by the HS extract, has the highest total phenolic content (38 ± 1.0 mg g−1 DE), with flavonoids as major components. The extracts showed remarkable enzyme inhibitory effects against cholinesterases (AChE and BChE – 0.56–2.03 mgGALAE per g extract), tyrosinase, and α-glucosidase. Only HO (61.32 ± 3.27 mgQE per g) and HP (39.88 ± 2.51 mgQE per g) extracts showed notable inhibitory potency against lipase, while all the tested extracts exhibited modest activity against α-amylase. The phytochemicals with the most favourable interactions in the enzymatic cavity of α-glucosidase were found to be hyperoside, myricetin, and neo-chlorogenic acid, each with a docking score of −7.7. From these results, the three Hypericum species can be classified as potent sources of biologically active secondary metabolites, which warrant further investigations as novel medicinal products.

Graphical abstract: Phytochemical characterization, in vitro and in silico approaches for three Hypericum species

Back to tab navigation

Publication details

The article was received on 20 Jan 2018, accepted on 05 Feb 2018 and first published on 06 Feb 2018


Article type: Paper
DOI: 10.1039/C8NJ00347E
Citation: New J. Chem., 2018,42, 5204-5214
  •   Request permissions

    Phytochemical characterization, in vitro and in silico approaches for three Hypericum species

    E. J. Llorent-Martínez, G. Zengin, D. Lobine, L. Molina-García, A. Mollica and M. F. Mahomoodally, New J. Chem., 2018, 42, 5204
    DOI: 10.1039/C8NJ00347E

Search articles by author

Spotlight

Advertisements