Study of interactions of mononucleotides with 1,4-dihydropyridine vesicles using NMR and ITC techniques

Abstract

The use of 1,4-dihydropyridine (DHP) vesicles as drug delivery agents can improve the diffusion of nucleoside and nucleotide prodrugs through the cellular membrane without degradation. Different NMR studies: spin–lattice relaxation in the rotating frame (T₁ρ), saturation transfer double difference (STDD), ¹H NMR chemical shift titration, as well as isothermal titration calorimetry (ITC) experiments, showed that mononucleotides bind to 1,4-DHP vesicles. The influence of the structure of the mononucleotides as well as that of the derivatives of 1,4-DHP on the complexation parameters was studied.