Affinity functionalization of PVA-co-PE nanofibrous membrane with Ni(ii)-chelated ligand for bovine hemoglobin adsorption

Abstract

We report that poly(vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane was surface functionalized by iminodiacetic acid (IDA) and then chelated by Ni(II) ions for hemoglobin adsorption. The results indicated that bovine hemoglobin (BHb) adsorption on Ni(II)-chelated PVA-co-PE nanofibrous membrane was better described by the Langmuir model. The experimental maximum adsorption capacity value was 66 mg g⁻¹. The method performed in this study provides an exploratory research for the large-scale purification of BHb and other proteins.