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An ultrasensitive electrochemical immunosensor based on C60-modified polyamidoamine dendrimer and Au NPs for co-catalytic silver deposition

Abstract

A convenient, high-efficient and ultrasensitive electrochemical immunosensor was developed in situ deposited silver nanoparticles (Ag NPs) as tracing tag for fetoprotein (AFP) detection. The strategy of the immunosensor was achieved by the co-catalytic effect on the surface of the nanocomposites containing polyamidoamine (PAMAM) dendrimer coated with C60 (PAMAM-C60 NPs) and gold nanoparticles (Au NPs). In addition, on the surface of Au@PAMAM-C60 NPs labeled antibody (Ab2), with the sanwich-type immunoreaction, the silver ions in the solution were reduced into metallic Ag NPs. The electrochemical signal in situ deposited Ag NPs could be utilized to monitor the immunoreaction. Under optimum conditions, peak current of Ag NPs increased with the increasing of AFP concentration in the range from 0. 1 pg mL-1 to 10 ng mL-1 with a limitation was 0.03 pg mL-1. Importantly, the designed electrochemical immunoassay strategy exhibited excellent sensitivity, stability, selectivity, and reproducibility.

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Publication details

The article was received on 05 Jan 2018, accepted on 12 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C8NJ00059J
Citation: New J. Chem., 2018, Accepted Manuscript
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    An ultrasensitive electrochemical immunosensor based on C60-modified polyamidoamine dendrimer and Au NPs for co-catalytic silver deposition

    P. Chen, T. Wang, X. Zheng, D. Tian, F. Xia and C. Zhou, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ00059J

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