Jump to main content
Jump to site search

Issue 8, 2018
Previous Article Next Article

Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine

Author affiliations

Abstract

In this article, the authors report a systematic study of the self-assembly of CdTe quantum dots (QDs) stabilized by mercaptosuccinic acid (MSA) at laboratory temperature (25 °C) or after thermal treatment (90 °C). It was found that QD/MSA can be synthesized spontaneously under mild laboratory conditions (25 °C and 70 hours) without any further instrumentation or they can be treated using an increased temperature (90 °C and 8 min) to achieve the same fluorescence intensity. These QDs were used for the preparation of a fluorescent (off–on) probe based on methylene blue as a quencher for the specific determination of nucleic acid from urine. The whole process was monitored using stationary spectroscopy (absorbance and fluorescence measurement) and capillary electrophoresis (CE) with laser-induced fluorescence detection. The average size of the prepared QDs (approximately 3 nm) was determined using transmission electron microscopy and it was proven that the presented fluorescent probe combined with the CE method can be used for the sensitive determination of DNA (LOD 3 ng mL−1).

Graphical abstract: Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine

Back to tab navigation

Publication details

The article was received on 05 Jan 2018, accepted on 06 Mar 2018 and first published on 09 Mar 2018


Article type: Paper
DOI: 10.1039/C7NJ05167K
Citation: New J. Chem., 2018,42, 6005-6012
  •   Request permissions

    Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine

    L. Nejdl, J. Zelnickova, T. Vaneckova, D. Hynek, V. Adam and M. Vaculovicova, New J. Chem., 2018, 42, 6005
    DOI: 10.1039/C7NJ05167K

Search articles by author

Spotlight

Advertisements