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Copper nanocluster as fluorescent indicator for label-free and sensitive detection of DNA hybridization assisted with cascade isothermal exponential amplification reaction

Abstract

Copper nanocluster (CuNCs)-based fluorescence biosensing platform was developed for label-free DNA detection through cascade isothermal exponential amplification reaction (EXPAR). It is based on target-triggered isothermal dual-cycling and a cascade EXPAR, which generates a large amount of long double-stranded DNA (dsDNA). The resultant long dsDNA can act as an efficient template for fluorescent CuNCs synthesis, and the produced CuNCs is an excellent fluorescence indicator to quantify target DNA concentration. This assay allows the detection of the model DNA with the detection limit of 7 pM. This label-free assay occurs in homogenous solution, and the whole assay process, including hybridization, amplification and detection, can be completed in a single-tube. This method is simple, low-cost and convenient to operate, and may offer potential applications in some related biomedical research.

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Publication details

The article was received on 06 Jan 2018, accepted on 11 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C7NJ05130A
Citation: New J. Chem., 2018, Accepted Manuscript
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    Copper nanocluster as fluorescent indicator for label-free and sensitive detection of DNA hybridization assisted with cascade isothermal exponential amplification reaction

    B. Li, X. Zhang and Y. Jin, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C7NJ05130A

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