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Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

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Abstract

This study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on silica microspheres (SiMP) with JEV as the template, (3-aminopropyl)triethoxysilane (APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed with SiMP, and the polymerization was performed in mild condition. After polymerization, templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV-MIPs can selectively recognize JEV in the presence of Hepatitis A virus (HAV), Simian virus 40 (SV40) and Rabies virus (RV), and also sensitively detect JEV with a picomolar detection limit.

Graphical abstract: Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

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Publication details

The article was received on 05 Dec 2017, accepted on 19 Jan 2018 and first published on 19 Jan 2018


Article type: Paper
DOI: 10.1039/C7NJ04791F
Citation: New J. Chem., 2018, Advance Article
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    Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

    W. Feng, C. Liang, H. Gong and C. Cai, New J. Chem., 2018, Advance Article , DOI: 10.1039/C7NJ04791F

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