Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

Abstract

This study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on silica microspheres (SiMP) with JEV as the template, (3-aminopropyl)triethoxysilane (APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed with SiMP, and the polymerization was performed in mild condition. After polymerization, templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV-MIPs can selectively recognize JEV in the presence of Hepatitis A virus (HAV), Simian virus 40 (SV40) and Rabies virus (RV), and also sensitively detect JEV with a picomolar detection limit.