Carbon dots derived from carboxymethylcellulose for sensing isoniazid and H2O2

Abstract

Herein, blue-fluorescence carbon dots (CDs) derived from carboxymethylcellulose have been innovatively synthesized with well-distributed sizes. Subsequently, a sensitive assay of isoniazid and H₂O₂ has been further proposed based on the hydrogen bond formation between CDs and isoniazid and the further oxidation of isoniazid by H₂O₂. To be specific, the richness of carboxyl groups and hydroxyl groups on the CDs enables isoniazid to be adsorbed on the surface of CDs, resulting in effective fluorescence quenching through aggregation. Moreover, we have taken the advantage of the oxidation reaction between isoniazid and H₂O₂ to achieve the immediate recovery of the fluorescence of CDs. Consequently, quantitative evaluation of H₂O₂ in a linear range from 4 nM to 800 nM has been proposed along with the range from 20 nM to 6 μM for isoniazid, and its practicability is subsequently validated by detecting actual samples, suggesting that the current strategy may broaden the avenues for sensing both isoniazid and H₂O₂.