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In-situ precipitation of hydrous titanium dioxide for dispersive micro solid-phase extraction of nucleosides and their separation

Abstract

Pure titanium dioxide (TiO2) adsorbent failed to selective capture of ribonucleosides in biological samples as previous works reported, although it was expected to display a specific recognition to cis-diol compounds by the Lewis acid sites on its surface. However, in this work, our results showed hydrous titania (TiO2•nH2O), that was obtained through hydrolyzation of tetrabutyl titanate (TBOT) in an aqueous solution, exhibited a high affinity to the cis-diol containing ribonucleosides for the first time. The adsorption efficiency of A, C and G by TiO2•nH2O can hold about 90 % even in a 100 % water matrix. On the other hand, the 2´-deoxynucleosides could only be adsorbed in a water-acetonitrile solution with the water content less than 10 %. Then an in-situ generated TiO2•nH2O-based dispersive micro solid-phase extraction (d-μ-SPE) was developed and evaluated. The whole d-μ-SPE process only takes about 7 minutes (2 min for extraction and 5 min for centrifugation) and no pre-synthesized or commercial TiO2 powder is required. The hydrous TiO2-based d-μ-SPE with a two-step elution strategy was successfully performed for extraction and separation of ribonucleosides (A, U) and 2´-deoxynucleosides (2´-dC, 2´-dG) in porcine plasma samples. The proposed TiO2•nH2O-based d-μ-SPE method is effective, simple and fast, and will have wide applications for selective extraction of cis-diol molecules in environmental and biological samples.

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Publication details

The article was received on 25 Nov 2017, accepted on 08 Feb 2018 and first published on 08 Feb 2018


Article type: Paper
DOI: 10.1039/C7NJ04590E
Citation: New J. Chem., 2018, Accepted Manuscript
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    In-situ precipitation of hydrous titanium dioxide for dispersive micro solid-phase extraction of nucleosides and their separation

    Y. Xu, Y. Yang, A. Xue, H. Chen and S. Li, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C7NJ04590E

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