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Rhodamine based turn-on chemosensor for Fe3+ in aqueous medium and interactions of its Fe3+ complex with DNA

Abstract

A novel di-coordinating rhodamine-based chemosensor, HL with NO donor atoms, selectively and rapidly recognizes Fe3+ in the presence of all biologically relevant as well as toxic metal ions and numerous anions. It exhibits lower detection limit (0.17µM) and comparatively high formation constant (Kf = 1.72 x 104 M–1). The DNA-binding properties of [L-Fe] 2+ complex have been comprehensively studied by using UV-Vis, fluorescence, optical melting studies and circular dichroism which clearly indicate that [L-Fe]2+ interacts with DNA via a groove binding mode. In particular, competition experiments with Hoechst and DAPI constitute firm evidence for this binding mode, and clearly rule out intercalation. The negative values of ΔG0 and positive entropy values obtained from calorimetric technique confirm the spontaneity of the binding of [L-Fe]2+ with DNA. These results clearly establish that [L-Fe]2+ can be used as a DNA groove binder and constitutes an advantageous substitute to some currently commercially available probes, such as Hoechst 33258 and DAPI. The resulting [L-Fe]2+/DNA composite material could be a valuable candidate for future photonics and/or biological applications.

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Publication details

The article was received on 20 Nov 2017, accepted on 11 Jan 2018 and first published on 12 Jan 2018


Article type: Paper
DOI: 10.1039/C7NJ04505K
Citation: New J. Chem., 2018, Accepted Manuscript
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    Rhodamine based turn-on chemosensor for Fe3+ in aqueous medium and interactions of its Fe3+ complex with DNA

    R. Bhowmick, A. S. M. Islam, U. Saha, G. Suresh Kumar and A. Mahammad, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C7NJ04505K

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