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Preparation and characterization of universal Fe3O4@SiO2/CdTe nanocomposites for rapid and facile detection and separation of membrane proteins

Abstract

Cell membrane protein has been recognized as a major marker for many diseases in clinical examination besides having many important physiological functions. The discovery of new cell membrane proteins has attracted much research interest. In the presented work, a multifunctional Fe3O4@SiO2/CdTe nanocomposite was designed for detection and separation of cell membrane protein, which was synthesized by covalent coupling reaction between amino groups and carboxyl groups. It could be serving as a universal tool for modifying antigens or other molecules having amino groups. In order to verify the feasibility and practicability of the method, a folic acid (FA)-functionalized magnetic/fluorescent nanoprobe (Fe3O4@SiO2/CdTe-NH-FA) has been developed for recognizing and separating the FA receptor on HepG2 cells. FA was fixed to the shell of Fe3O4@SiO2/CdTe nanocomposites via 1,6-hexamethylenediamine. It obviously reduces the fluorescence quenching effect of FA on magnetic/fluorescent nanocomposites. The results demonstrated that the synthesized nanoprobes, Fe3O4@SiO2/CdTe-NH-FA, provided an excellent optical characteristic to make reliable imaging of cancer cells and good magnetic properties to enrich and isolate cell membrane protein. As a consequence, this biological nanoprobe could be used to find, obtain and study unknown membrane proteins and predict the occurrence of some diseases. And this nanoprobe will be a potential platform for cancer accurate diagnosis and therapy in the future.

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Publication details

The article was received on 21 Nov 2017, accepted on 08 Feb 2018 and first published on 09 Feb 2018


Article type: Paper
DOI: 10.1039/C7NJ04484D
Citation: New J. Chem., 2018, Accepted Manuscript
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    Preparation and characterization of universal Fe3O4@SiO2/CdTe nanocomposites for rapid and facile detection and separation of membrane proteins

    M. Wang, K. Zheng, S. Lv, H. Zou, H. Liu, G. Yan, A. Liu and X. Fei, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C7NJ04484D

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