Jump to main content
Jump to site search


A novel glucosamine-linked fluorescence chemosensor for the detection of pyrophosphate in aqueous medium and live cells

Abstract

There is a growing interest in monitoring the level of pyrophosphate (PPi) in biological processes due to its significant roles in live organisms. In this paper, we described a Cu2+ ensemble based chemosensor (GN-Cu2+) for highly selective detection of PPi in aqueous medium and live cells. The fluorescence silent ensemble, GN-Cu2+ was facilely prepared by complexing of the glucosamine-1,8-naphthalimide fluorescent ligand (GN) with paramagnetic Cu2+. The structure of GN was confirmed by 1H NMR, 13C NMR and high-resolution mass spectroscopy (HRMS) assays. The spectroscopic characteristics of GN-Cu2+ were verified in detail through fluorescence emission and UV-vis spectra titration. The decomplexation of GN-Cu2+ by PPi led to the liberation of the fluorescent ligand, GN, and thus the fluorescence was switched on. Spectroscopic studies revealed that GN-Cu2+ possesses excellent tolerance to various anions and even other phosphate series, such as AMP, ADP, ATP, and inorganic phosphate (Pi). Detection limit for PPi was calculated to be 1.0 µM. GN-Cu2+ features several advantages, such as high selectivity, reliability at physiological pH and low cytotoxicity. Intracellular fluorescence imaging measurements indicated that GN-Cu2+ is cell membrane permeable and has been successfully used for detection of PPi in live MD-AMB-231 cells.

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Oct 2017, accepted on 06 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7NJ04107A
Citation: New J. Chem., 2018, Accepted Manuscript
  •   Request permissions

    A novel glucosamine-linked fluorescence chemosensor for the detection of pyrophosphate in aqueous medium and live cells

    Q. Meng, Y. Wang, H. Feng, F. Zhou, B. Zhou, C. Wang, R. Zhang and Z. Zhang, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C7NJ04107A

Search articles by author

Spotlight

Advertisements