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Synthesis, characterization and in vitro screening of a nano-hydroxyapatite/chitosan/Euryale ferox nanoensemble – an inimitable approach for bone tissue engineering

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Abstract

In order to explore novel synthetic bone scaffolds as part of our ongoing research in the field of bone tissue engineering, we have synthesized a biomimmetic, osteoinductive, tricomposite scaffold incorporating Euryale ferox (EF) with nano-hydroxyapatite and chitosan. The surface morphology of the resultant nanocomposite was visualized via SEM and TEM studies which exhibited a rougher surface of n-HA/CS/EF compared to the n-HA/CS nanocomposite with the particle size ranging between 15 and 20 nm. The n-HA/CS/EF scaffold was degraded up to (16.9 ± 0.70)% in contrast to degradation of (7.4 ± 0.2)% for n-HA/CS. The in vitro SBF study exhibited excellent biomineralization capacity for n-HA/CS/EF which was further elucidated using the ARS staining procedure. The MTT assay illustrated significantly better cell viability compared to the n-HA/CS nanocomposite. A comparatively better antibacterial activity of n-HA/CS/EF was observed against a number of bacterial pathogens. The osteogenic differentiation test (ALP assay) assessed the osteoconductivity of the n-HA/CS/EF nanocomposite. Thus, the synthesized scaffold can be envisioned for potential use in reparation of bone defects.

Graphical abstract: Synthesis, characterization and in vitro screening of a nano-hydroxyapatite/chitosan/Euryale ferox nanoensemble – an inimitable approach for bone tissue engineering

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Publication details

The article was received on 09 Aug 2017, accepted on 17 Nov 2017 and first published on 17 Nov 2017


Article type: Paper
DOI: 10.1039/C7NJ02953E
Citation: New J. Chem., 2018, Advance Article
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    Synthesis, characterization and in vitro screening of a nano-hydroxyapatite/chitosan/Euryale ferox nanoensemble – an inimitable approach for bone tissue engineering

    M. Shakir, S. Mirza, R. Jolly, A. Rauf and M. Owais, New J. Chem., 2018, Advance Article , DOI: 10.1039/C7NJ02953E

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