Jump to main content
Jump to site search

Cardiac myoglobin participates in the metabolic pathway of selenium in rat


An essential micronutrient selenium deficiency is a leading-cause of cardiovascular diseases. The heart is continuously beating for blood delivery to the entire body, which requires a high-energy demand. The adult heart normally obtains 50–70% of its adenosine 5’-triphosphate from fatty acid β-oxidation. An increase in the fatty acid oxidation activity induces the generation of larger amounts of by-products (reactive oxygen species, ROS) from mitochondrial oxidative phosphorylation. Selenium-dependent glutathione peroxidases play the critical role for removal of the ROS, especially organic hydroperoxides, from the heart. The definitive transport and/or detailed metabolic pathways from the selenium-source compounds to the selenoproteins in the heart still remain unclear. We explored the selenium-binding proteins in the rat cardiac cell lysate using its reactive metabolic intermediate selenotrisulfide (STS) and MALDI TOF-mass spectrometry. Several proteins with the free cysteine (Cys) thiol were found to be reactive with STS through the thiol-exchange reaction. The most distinctive Cys-containing protein in the cardiac cell lysate was identified as myoglobin (Mb) from a rat protein database search and tryptic fragmentation experiments. When separately examined in selenium-adequate rats, selenium-binding to the cardiac Mb was verified by the selenium-specific fluorometry. Cardiac Mb was thought to participate in the selenium metabolic pathway in the heart.

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Jan 2018, accepted on 09 Mar 2018 and first published on 09 Mar 2018

Article type: Paper
DOI: 10.1039/C8MT00011E
Citation: Metallomics, 2018, Accepted Manuscript
  •   Request permissions

    Cardiac myoglobin participates in the metabolic pathway of selenium in rat

    M. HARATAKE, E. Hori, S. YOSHIDA, T. FUCHIGAMI and M. Nakayama, Metallomics, 2018, Accepted Manuscript , DOI: 10.1039/C8MT00011E

Search articles by author