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Embryonic exposure to an aqueous coal dust extract results in gene expression alterations associated with development and function of connective tissue and the hematological system, immunological and inflammatory disease, and cancer in zebrafish

Abstract

Coal mining is one of the economic activities with the greatest impacts on environmental quality. At all stages contaminants are released as particulates such as coal dust. The first aim of this study was to obtain an aqueous coal dust extract and characterize its composition in terms of trace elements by ICP-MS. In addition, the developmental toxicity of the aqueous coal extract was evaluated using the zebrafish (Danio rerio) after exposure to different concentrations (0-1000 ppm; μg/mL) to establish acute toxicity, morphology and transcriptome changes. Trace elements within the aqueous coal dust extract yielding the highest concentrations (>10 ppb) included Sr, Zn, Ba, As, Cu and Se. In addition, Cd and Pb were found in lower concentrations. No significant difference in mortality was observed (p>0.05), but a delay in hatching was found at 0.1 and 1000 ppm (p<0.05). No significant differences in morphological characteristics were observed in any of the treatment groups (p>0.05). Transcriptomic results of zebrafish larvae revealed alterations in 77, 61 and 1,376 genes in the 1, 10, and 100 ppm groups, respectively. Gene ontology analysis identified gene alterations associated with development and function of connective tissue and the hematological system, as well as pathways associated with apoptosis, cell cycle, transcription, and oxidative stress including the MAPK signaling pathway. In addition, altered genes were associated with cancer; connective tissue, muscular, and skeletal disorders; and immunological and inflammatory disease. Overall, this is the first study to characterize gene expression alterations in response to a developmental exposure to aqueous coal dust residue from coal mining with transcriptome results signifying functions and systems to target in future studies.

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Supplementary files

Publication details

The article was received on 27 Oct 2017, accepted on 15 Feb 2018 and first published on 15 Feb 2018


Article type: Paper
DOI: 10.1039/C7MT00300E
Citation: Metallomics, 2018, Accepted Manuscript
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    Embryonic exposure to an aqueous coal dust extract results in gene expression alterations associated with development and function of connective tissue and the hematological system, immunological and inflammatory disease, and cancer in zebrafish

    K. Caballero-Gallardo, S. E. Wirbisky, J. Olivero-Verbel, J. de la Rosa and J. L. Freeman, Metallomics, 2018, Accepted Manuscript , DOI: 10.1039/C7MT00300E

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